nih3t3 embryonic swiss murine fibroblast cell line atcc crl 1658  (ATCC)

Journal: Cells

Article Title: Atypical Exon 2/3 Mutants G48C, Q43K, and E37K Present Oncogenic Phenotypes Distinct from Characterized NRAS Variants

doi: 10.3390/cells13201691

Figure Lengend Snippet: Effect of the novel NRAS mutants on cell proliferation. NRAS G48C, Q43K, and E37K enhanced proliferation of both HCT116 and NIH3T3 cells compared to vector-only, wild-type, and canonical mutant control Q61K. Proliferation rates of ( a ) HCT116 cells and ( b ) NIH3T3 cells transfected with empty vector, wild-type NRAS, canonical NRAS Q61K mutant, and the novel NRAS mutants G48C, Q43K, and E37K are shown. Data presented are representative of three independent trials, each performed in triplicate, and expressed as mean ± standard deviation (SD). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. PTT = pTargeT vector-only control; WT = wild-type; ns = non-significant.

Article Snippet: Two cell lines were used in this study, as follows: the NIH3T3 murine embryonic fibroblast cell line (Cat. No. CCL-247) and the HCT116 human colorectal carcinoma cell line (Cat. No. CCL-247), which were both obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Plasmid Preparation, Mutagenesis, Control, Transfection, Standard Deviation

Journal: Cells

Article Title: Atypical Exon 2/3 Mutants G48C, Q43K, and E37K Present Oncogenic Phenotypes Distinct from Characterized NRAS Variants

doi: 10.3390/cells13201691

Figure Lengend Snippet: Effect of the novel NRAS mutants, G48C, Q43K, and E37K, on cell survival. ( a ) HCT116 and ( b ) NIH3T3 cells were transfected with NRAS WT and mutant constructs, then assessed for their ability to resist apoptosis when induced with sodium butyrate (HCT116) and reduced serum concentration (NIH3T3). Data presented are representative of three independent trials, each performed in triplicate, and expressed as mean ± standard deviation (SD). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. PTT = pTargeT vector-only control; WT = wild-type; ns = non-significant.

Article Snippet: Two cell lines were used in this study, as follows: the NIH3T3 murine embryonic fibroblast cell line (Cat. No. CCL-247) and the HCT116 human colorectal carcinoma cell line (Cat. No. CCL-247), which were both obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Transfection, Mutagenesis, Construct, Concentration Assay, Standard Deviation, Plasmid Preparation, Control

Journal: Cells

Article Title: Atypical Exon 2/3 Mutants G48C, Q43K, and E37K Present Oncogenic Phenotypes Distinct from Characterized NRAS Variants

doi: 10.3390/cells13201691

Figure Lengend Snippet: Effect of NRAS G48C, Q43K, and E37K mutants on cellular migration in HCT116 and NIH3T3 cells. Scratch wound assay showing representative images of ( a ) HCT116 and ( c ) NIH3T3 cells transfected with empty vector, wild-type NRAS, canonical NRAS Q61K control, and the novel NRAS mutants G48C, Q43K, and E37K at 0 h and 16 h post-scratch. The migration rates for each setup are shown in ( b , d ) for HCT116 and NIH3T3 cells, respectively. Data presented are representative of three independent trials, each performed in triplicate, and expressed as mean ± standard deviation (SD). ** p ≤ 0.01. pTT = pTargeT vector-only control; WT = wild-type; ns = non-significant.

Article Snippet: Two cell lines were used in this study, as follows: the NIH3T3 murine embryonic fibroblast cell line (Cat. No. CCL-247) and the HCT116 human colorectal carcinoma cell line (Cat. No. CCL-247), which were both obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Migration, Scratch Wound Assay Assay, Transfection, Plasmid Preparation, Control, Standard Deviation

Journal: Cells

Article Title: Atypical Exon 2/3 Mutants G48C, Q43K, and E37K Present Oncogenic Phenotypes Distinct from Characterized NRAS Variants

doi: 10.3390/cells13201691

Figure Lengend Snippet: Expression of NRAS mutants induce cytoskeletal remodeling in NIH3T3 cells. Distinct features, such as prominent peripheral stress fibers (yellow arrows) and highly organized actin filaments (red arrowheads), are present in both the empty vector ( A ) and wild-type ( B ) setups. Central round nuclei (orange arrowheads) are also visible in WT. Changes in cytoskeletal architecture, as well as invasive structures, are observable in both canonical and novel mutant setups ( C , D = Q61K; E , F = G48C; G , H = Q43K; I , J = E37K), including fan-shaped cells with prominent migrating front (sky blue brackets); peripheral dorsal ruffles (pink arrowheads); circular dorsal ruffles (yellow arrowheads); tunneling nanotubes (gray arrowheads); multilobulated nuclei (red arrows); invadopodia (pink arrows); lamellipodia (gold arrowheads); filopodia (purple arrowheads); and fusing cells (yellow brackets).

Article Snippet: Two cell lines were used in this study, as follows: the NIH3T3 murine embryonic fibroblast cell line (Cat. No. CCL-247) and the HCT116 human colorectal carcinoma cell line (Cat. No. CCL-247), which were both obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Plasmid Preparation, Mutagenesis

Journal: Heliyon

Article Title: Houttuynia cordata Thunb. Hexane fraction induces MDA-MB-231 cell apoptosis via caspases, ER stress, cell cycle arrest and attenuated Akt/ERK signaling

doi: 10.1016/j.heliyon.2023.e18755

Figure Lengend Snippet: Cytotoxicity of H. cordata crude extract and H fraction against human breast cancer MDA-MB-231 and MCF-7 cell lines, normal murine embryonic fibroblast NIH3T3 cell line, and human peripheral blood mononuclear cells (PBMCs). The percentage cell viability of these cells is shown on the Y-axis. The percentage of cell viability was determined after treatment with crude extract (A), and hexane fraction (B) for 24 h at various concentrations as shown on the X-axis. The data are reported as mean ± S.D. of triplicate from three independent experiments. The significance of statistical values is marked with * p < 0.05, **p < 0.01 , *** p < 0.001 vs control.

Article Snippet: The human breast cancer MDA-MB-231, MCF-7 and NIH3T3 murine embryonic fibroblast cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control

Journal: Heliyon

Article Title: Houttuynia cordata Thunb. Hexane fraction induces MDA-MB-231 cell apoptosis via caspases, ER stress, cell cycle arrest and attenuated Akt/ERK signaling

doi: 10.1016/j.heliyon.2023.e18755

Figure Lengend Snippet: Summary of the 50% inhibitory concentration (IC 50 ) of crude extract and each fraction on MDA-MB-231, MCF-7, NIH3T3 cells, and PBMCs.

Article Snippet: The human breast cancer MDA-MB-231, MCF-7 and NIH3T3 murine embryonic fibroblast cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Concentration Assay

Journal: Heliyon

Article Title: Houttuynia cordata Thunb. Hexane fraction induces MDA-MB-231 cell apoptosis via caspases, ER stress, cell cycle arrest and attenuated Akt/ERK signaling

doi: 10.1016/j.heliyon.2023.e18755

Figure Lengend Snippet: Selectivity index (SI) of HCT H fraction in BC cells compared to normal cells.

Article Snippet: The human breast cancer MDA-MB-231, MCF-7 and NIH3T3 murine embryonic fibroblast cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Biomedical Optics Express

Article Title: Method for nanoparticles uptake evaluation based on double labeled fluorescent cells scanned in enhanced darkfield microscopy

doi: 10.1364/BOE.490136

Figure Lengend Snippet: a) Fluorescence image of a group of NIH3T3 cells (experimental colors through F4 filter); b) cross-section from F1-Z-stack; c) cross-section from F2-Z-stack; d) deconvolved image of b; e) deconvolved image of c; f) cross-section from WL-Z-stack with localized ZnO-NPs; g)-i) movie frames obtained by combining all Z-stacks (digital colors).

Article Snippet: Murine embryonic fibroblast cell line NIH3T3 (ATCC-CRL 1658) was used.

Techniques: Fluorescence

Journal: Biomedical Optics Express

Article Title: Method for nanoparticles uptake evaluation based on double labeled fluorescent cells scanned in enhanced darkfield microscopy

doi: 10.1364/BOE.490136

Figure Lengend Snippet: The metabolic viability of NIH3T3 cells incubated for 24 h with different concentrations of ZnO-NPs.

Article Snippet: Murine embryonic fibroblast cell line NIH3T3 (ATCC-CRL 1658) was used.

Techniques: Incubation

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: Anti-cancer Effect and Active Phytochemicals of Houttuynia cordata Thunb. against Human Breast Cancer Cells

doi: 10.31557/APJCP.2023.24.4.1265

Figure Lengend Snippet: H. cordata Crude Extract and Different Fractions' Cytotoxic Effects on the Normal NIH3T3 Murine Embryonic Fibroblast Cell Line as Well as the Breast Cancer MDA-MB-231 and MCF-7 Cell Lines. After 24 hours of exposure to crude extract (A), hexane (B), dichloromethane (C), ethyl acetate (D), and methanol (E) fractions at varied concentrations, the percentage of cell viability was calculated. The data are shown as the mean ± SD of three independent trials done in triplicate. The statistical significance of values is indicated by the symbols *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Cell lines and cell culture The American Type Culture Collection provided the human breast cancer MDA-MB-231, MCF-7 and murine embryonic fibroblast NIH3T3 cells (ATCC, Manassas, VA, USA).

Techniques: